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For example, shapes of envelope projections or nucleoprotein complexes and the presence or absence of virus cores can prove useful and in some cases decisive for correct identification of the observed virus particles. 3. Symmetries associated with viruses Crick and Watson (1956) suggested that coats of small viruses are built up of identical protein molecules packed together in a regular way. Both theoretical considerations and experimental studies which followed showed that there are only a limited number of possible designs for a biological ‘container’ to be constructed from identical building blocks (Caspar and Klug, 1962, 1963; Caspar, 1980).
Ultrastruct. Res. 47, 361-383. W. and Wildy P. (1979) An historical account of the development and applications of the negative staining technique to the electron microscopy of viruses. J. Microsc. 117, 103-122. A. (1978) Electron microscopy of specific proteins: three-dimensional mapping of ribosomal proteins using antibody labels. In Advanced Techniques in Biological Electron Microscopy 11, Ed. K. Koehler, pp. 173-21 1. Springer-Verlag, Berlin. V. (1977) Freeze-drying for electron microscopy.
Excess stain is usually removed by touching the grid to the blotting paper. Transferring grids from drop to drop with forceps can break thin supporting films; 23 G F Fig. 1. Diagram of the negative staining-carbon film technique. A: freshly cleaved mica 2 X 1 cm pointed. B: mixture of virus (1-2 mg/ml) suspension and 3 % ammonium molybdate spread onto surface. C: excess liquid drained with filter paper. Specimen air-dried. D: very thin film of carbon evaporated onto specimen. E: mark placed on carbon to identify specimen.